hammerhead$33585$ - перевод на голландский
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hammerhead$33585$ - перевод на голландский

CATALYZES REVERSIBLE CLEAVAGE AND LIGATION WITHIN AN RNA MOLECULE
Hammerhead ribozyme (type I); Hammerhead ribozyme (type III); Hammerhead ribozyme type III; Hammerhead ribozyme type I; Hammerhead RNA; Hammerhead Ribozyme
  • The active site of the full-length hammerhead ribozyme. G12 is positioned consistent with a role as a general base in the cleavage reaction, and the 2'-OH of G8 is positioned for acid catalysis. Potentially "active" hydrogen bonds are shown as orange dotted lines. The 2'-O of C17 is shown to be aligned for nucleophilic attack along the blue dotted line trajectory.
  • Three-dimensional structure of the full-length hammerhead ribozyme
  • In-line transition-state for the hammerhead ribozyme reaction. A general base (B) abstracts a proton from the 2'-O, and a general acid (A), supplies a proton to the 5'-O leaving group as negative charge accumulates. The reaction product is a 2',3'-cyclic phosphate. The bonds breaking (shown in red) and forming (dashed lines) must be in the axial positions and reside approximately 180° apart, as shown. Electron movements are represented by curly arrows.
  • Possible transition-state interactions as extrapolated from the crystal structure.
  • Secondary structures and sequences of the minimal (A) and full-length (B) hammerhead ribozymes. Conserved and invariant nucleotides are shown explicitly. Watson-Crick base-paired helical stems are represented as ladder-like drawings. The red arrow depicts the cleavage site, 3' to C17, on each construct.
  • The crystal structure of a minimal hammerhead ribozyme

hammerhead      
n. hamerhaai

Определение

Hammerhead
·noun A fresh-water fish; the stone-roller.
II. Hammerhead ·noun An African fruit bat (Hypsignathus monstrosus);
- so called from its large blunt nozzle.
III. Hammerhead ·noun A shark of the genus Sphyrna or Zygaena, having the eyes set on projections from the sides of the head, which gives it a hammer shape. The Sphyrna zygaena is found in the North Atlantic. Called also hammer fish, and balance fish.

Википедия

Hammerhead ribozyme

The hammerhead ribozyme is an RNA motif that catalyzes reversible cleavage and ligation reactions at a specific site within an RNA molecule. It is one of several catalytic RNAs (ribozymes) known to occur in nature. It serves as a model system for research on the structure and properties of RNA, and is used for targeted RNA cleavage experiments, some with proposed therapeutic applications. Named for the resemblance of early secondary structure diagrams to a hammerhead shark, hammerhead ribozymes were originally discovered in two classes of plant virus-like RNAs: satellite RNAs and viroids. They are also known in some classes of retrotransposons, including the retrozymes. The hammerhead ribozyme motif has been ubiquitously reported in lineages across the tree of life.

The self-cleavage reactions, first reported in 1986, are part of a rolling circle replication mechanism. The hammerhead sequence is sufficient for self-cleavage and acts by forming a conserved three-dimensional tertiary structure.